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Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production

✍ Scribed by Seung Bum Sohn; Alexandra B. Graf; Tae Yong Kim; Brigitte Gasser; Michael Maurer; Pau Ferrer; Diethard Mattanovich; Sang Yup Lee

Book ID
112012233
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
646 KB
Volume
5
Category
Article
ISSN
1860-6768

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Genome-scale analysis of library sorting
Genome-scale analysis of library sorting (GALibSo): Isolation of secretion enhancing factors for recombinant protein production in Pichia pastoris
✍ Gerhard Stadlmayr; Katrin Benakovitsch; Brigitte Gasser; Diethard Mattanovich; M 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 743 KB

## Abstract A method combining fluorescence activated cell sorting (FACS) and DNA microarray assisted clone identification was developed and termed Genome‐Scale Analysis of Library Sorting (GALibSo). Genes enhancing the production of secreted heterologous proteins in __Pichia pastoris__ were identi