The mitochondrial genome from Cyprinus carpio oocytes is a 10.5 megadalton, circular DNA molecule. The carp mitochondrial DNA was cloned in pBR325. Three recombinant plasmids accounted for the entire genome. Mapping of this DNA using 11 different restriction endonucleases is reported here. Both the
Genome organization of mitochondrial DNA from the non-saccharomycete yeastArxula adeninivoransLS3
โ Scribed by Uta Pich; Gotthard Kunze
- Publisher
- Springer-Verlag
- Year
- 1992
- Tongue
- English
- Weight
- 395 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0172-8083
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โฆ Synopsis
Mitochondrial (mt) DNA of the asexual ascomycetous yeast Arxula adeninivorans LS3 was isolated and characterized. The mtDNA has a GC content of 30.3 mol%. It is circular and its size, as estimated by restriction analysis performed with nine endonucleases, was 35.5 kbp. Using mt gene-probes from Saccharomyces cerevisiae six structural genes (cob, cox1, cox2, oli1, oli2, and 21S rRNA) were located on the mitochondrial genome of A. adeninivorans. The comparison between the mt genomes of A. adeninivorans and other yeasts showed differences in genome organization.
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