Genetics of somatic mammalian cells. XV. Evidence for linkage between human genes for lactic dehydrogenase B and serine hydroxymethylase

✍ Scribed by Carol Jones; Paul Wuthier; Fa-Ten Kao; Theodore T. Puck

• Publisher: John Wiley and Sons
• Year: 1972
• Tongue: English
• Weight: 550 KB
• Volume: 80
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040800217

✦ Synopsis

Chincsc hamster ovary cells with a deficiency i n Serine Hydroxymethylase which produces a specific glycine auxotrophy (gly-A) were fused with human cells from a variety of sources and the resulting hybrids analyzed for human gene linkage. Of 102 hybrid clones examined 65 possessed both glyA and lactic dehydrogenase B markers, 35 possessed neither marker. Two clones were found with altered gIycine responses which were not linked to LDH-B. The data indicate linkagc between genes responsible for serine hydroxymethylase activity and lactic dehydrogenase B. Evidencc: for. absence of linkage between these arid a variety of other g e m s is also presented.