Cover......Page 1
Half Title......Page 2
Series Page......Page 3
Title Page......Page 4
Copyright Page......Page 5
Dedication......Page 6
Introduction to the Series......Page 7
Preface......Page 8
Table of Contents......Page 12
Contributors......Page 15
I: Strategies and Techniques......Page 20
1. General Methods......Page 22
2. Cloning Strategies......Page 62
3. Protein Engineering Strategies......Page 74
4. General Strategies in In Vivo Animal Modeling......Page 82
II: Ricin......Page 98
5. Molecular Cloning of Ricin......Page 100
6. Expression of Plant-Derived Ribosome-Inactivating Proteins in Heterologous Systems......Page 118
7. The Structure of Plant Toxins as a Guide to Rational Design......Page 152
8. Chemical and Genetic Characterization of the Enzymatic Activity Associated with Ricin A Chain......Page 170
9. Chimeric Proteins Containing Ricin A Chain......Page 202
III: Plant Hemitoxins......Page 210
10. Studies on Ribosome-Inactivating Proteins from Saponaria officinalis......Page 212
11. Cloning and Expression of Trichosanthin and a-Momorcharin cDNA......Page 232
12. Cloning and Expression of a Luffa Ribosome-Inactivating Protein-Related Protein......Page 242
IV: Fungal Ribotoxins......Page 254
13. The Aspergillus Ribonucleolytic Toxins (Ribotoxins)......Page 256
14. An Efficient Expression System for a-Sarcin in Escherichia coli......Page 278
V: Diphtheria Toxin......Page 290
15. Diphtheria Toxin Cloning and Expression in Corynebacterium diphtheriae......Page 292
16. Diphtheria Toxin Expression in Escherichia coli......Page 326
17. The Structure of Diphtheria Toxin as a Guide to Rational Design......Page 358
18. Protein Engineering of Diphtheria Toxin: Development of Receptor-Specific Cytotoxic Agents for the Treatment of Human Disease......Page 384
19. In Vivo Studies with Chimeric Toxins: Interleukin-2 Fusion Toxins as Immunosuppressive Agents......Page 402
20. Initial Clinical Experiences with an lnterleukin-2 Fusion Toxin (DAB-IL-2)......Page 414
VI: Pseudomonas Exotoxins......Page 422
21. Pseudomonas aeruginosa Exotoxin A......Page 424
22. Expression of Growth Factor-Toxin Fusion Proteins......Page 444
23. The Structure of Pseudomonas Exotoxin A as a Guide to Rational Design......Page 458
24. Generation of Chimeric Toxins......Page 466
VII: Conclusions......Page 482
25. Genetically Engineered Toxins in Perspective......Page 484
Appendix: Primary Amino Acid Sequences of Toxins......Page 498
Index......Page 504
