Genetic Variation of a Collagen IV α1-Chain Gene Polymorphism in Danish Insulin-dependent Diabetes Mellitus (IDDM) Patients: Lack of Association to Nephropathy and Proliferative Retinopathy
✍ Scribed by Chen, J.-W.; Hansen, P.M.; Tarnow, L.; Hellgren, A.; Deckert, T.; Pociot, F.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 123 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0742-3071
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✦ Synopsis
In insulin-dependent (Type 1) diabetes mellitus (IDDM) the development of nephropathy is partly due to genetic susceptibility. Previously one study has demonstrated a relationship between a HindIII restriction polymorphism of the collagen IV ␣1-chain gene and diabetic nephropathy. The aim of the present study was to evaluate such as association in a casecontrol study including 207 Danish IDDM patients: 116 with nephropathy (urinary albumin excretion rate (AER) Ͼ Ͼ Ͼ 300 mg 24 h ؊1 ) and 91 without nephropathy (AER Ͻ Ͻ Ͻ 30 mg 24 h ؊1 ). Using genomic DNA, HindIII restriction fragment length analysis revealed a bi allele polymorphism visualized by Southern hybridization with a cDNA probe recognizing the collagen IV ␣1-chain gene. No differences in genotype frequencies or allele frequencies were demonstrated comparing patients with and without nephropathy: p = 0.39 and p = 0.96, respectively. Neither were there any difference in genotype frequencies or allele frequencies when the patients were stratified according to the presence of proliferative retinopathy: p = 0.44 and p = 0.84, respectively. Pooling the diabetic groups revealed genotype frequencies and allele frequencies comparable to those found in 57 healthy unrelated Danish individuals. We conclude that in a Danish IDDM population a HindIII restriction polymorphism of the collagen IV ␣1-chain gene is not associated with diabetic nephropathy, diabetic retinopathy or with diabetes per se.