Genetic variation in natural populations of maté (Ilex paraguariensis A. St.-Hil., Aquifoliaceae) using RAPD markers
✍ Scribed by Luciane Gauer; Suzana Cavalli-Molina
- Publisher
- Nature Publishing Group
- Year
- 2000
- Tongue
- English
- Weight
- 467 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0018-067X
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✦ Synopsis
This study characterized the genetic diversity of four populations of Ilex paraguariensis, a plant species native to South America, using random amplified polymorphic DNA (RAPD) markers. A total of 341 different RAPD bands were generated by the 15 primers analysed. High genetic variability was detected within each population, with an average diversity of 0.163. The within-population variation was large, probably as a result of the life history characteristics of I. paraguariensis. The average distance between individuals from each population was 0.392 and that between populations was 0.433, indicating a low between-population divergence. Most bands were common to all populations and the population-specific bands occurred at low frequencies. Partitioning of the genetic diversity indicated that 85% of the variation occurred within populations and only 15% between populations. Although most of the variability is within populations, clusterings of plants in each population were observed in the dendrogram.