Genetic variability in the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase and its small subunit mRNA in pea
β Scribed by Shaun L. A. Hobbs; Lawrence E. Pelcher; Catherine M. O. DeLong; Maureen Anderson; John D. Mahon
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 788 KB
- Volume
- 180
- Category
- Article
- ISSN
- 0032-0935
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A mutant of the small subunit of ribulose-l,5-bisphosphate carboxylase/oxygenase (Rubisco), in which Arg53 is replaced by Glu, was synthesized and imported into isolated chloroplasts. The mutant protein was efficiently imported into the chloroplast and correctly processed to the mature size. Like th
The small subunit (S SU) of Rubisco is synthesized in the cytosol in a precursor form. Upon import into the chloroplast, it is proteolytically processed at a Cys-Met bond to yield the mature form of the protein. To assess the importance of the Met residue for recognition and processing by the stroma