๐”– Bobbio Scriptorium
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Genetic transformation ofMedicagospecies byAgrobacterium tumefaciensand electroporation of protoplasts

โœ Scribed by Nicolay Kuchuk; Igor Komarnitski; Anatoliy Shakhovsky; Yuri Gleba

Publisher
Springer
Year
1990
Tongue
English
Weight
682 KB
Volume
8
Category
Article
ISSN
0721-7714

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โœฆ Synopsis

Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA in to the genome of the regenerated plants.Transgenic plants resistant to kanamycin were obtained by electroporation of Medicago borealis protoplasts with the pGA 472 plasmid DNA.

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