Genetic transformation of Pseudomonas oleovorans by electroporation
โ Scribed by Daniel K.Y. Solaiman
- Book ID
- 110269354
- Publisher
- Springer-Verlag
- Year
- 1998
- Tongue
- English
- Weight
- 218 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0951-208X
No coin nor oath required. For personal study only.
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A method was developed for the introduction of plasmids into Clostridium botulinurn by electroporation. A 4.4 kb plasmid vector, pGK12, which contains genes for resistance to erythromycin (Em') and chloramphenicol (Cmr) was electroporated into C. botulinurn type A (Hall A). The highest transformatio