Genetic studies of the ribosomal proteins in Escherichia coli XI
โ Scribed by Takata, Renkichi
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 454 KB
- Volume
- 160
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
E. coli episomes which cover argG region were transferred to S. marcescens and ribosomal proteins (r-proteins) from these hybrid strains were analyzed by two-dimensional (2D) polyacrylamide gel electrophoresis. Three E. coli r-proteins L21, L27 and S15 could be detected in the ribosomes from the hybrid strains. The relative rate of synthesis of the individual r-proteins were determined for E. coli merodiploid strains harboring these episomes. Over-production of three r-proteins L21, L27 and S21 (but not S15) was observed in the merodiploid strains.
๐ SIMILAR VOLUMES
Episomes of E. coli, which cover argG but not the str region, were transferred to Serratia marcescens. Ribosomal proteins from these hybrid strains were analyzed with phospho-cellulose or carboxy-methyl-cellulose column chromatography. Two E. coli ribosomal proteins, L21 and S15, could be detected i