𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Genetic segregation in a high-yielding streptomycin-producing strain of Streptomyces griseus

✍ Scribed by Dr. M. Roth; B. Schwalenberg; R. Reiche; D. Noack; R. Geuther; I. Eritt

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
474 KB
Volume
22
Category
Article
ISSN
0233-111X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The streptomycin‐producing Streptomyces griseus HP spontaneously segregated non‐reverting derivatives with altered phenotypes. Clones characterized by increased spore formation and decreased streptomycin production were found. Two other types of derivatives were defective in aerial mycelium and streptomycin formation as well, but differed in the capacity to synthesize a yellow pigment. These derivatives were examined with respect to further properties.

The stability of S. griseus HP was investigated in relation to conditions of continuous culture. Both at 26 and 30°C, under glycerol and NH~4~Cl limitation a rapid segregation and enrichment of streptomycin‐non‐producing derivatives occurred. At 34°C and glycerol limitation segregation began only after about 35 generations of continuous culture. In NH~4~Cl‐limited chemostats the original strain was stable during 80 generations. In the course of the continuous culture experiments it was shown that the onset of genetic segregation with mycelia can be detected before it becomes obvious in colonies grown from the mycelia. This was achieved by fractionation of the mycelia by protoplast formation and subsequent plating on regeneration medium allowing colony growth and differentation.

📜 SIMILAR VOLUMES

Biochemical characteristics of non-strep
Biochemical characteristics of non-streptomycin-producing mutants of Streptomyces griseus I. Role of NAD (P)-glycohydrolase in cell differentiation
✍ Dr. U. Gräfe; M. Roth; A. Christner; E. J. Bormann 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 674 KB

## Abstract Five non‐streptomycin‐producing mutants of an industrial strain of __Streptomyces griseus__ lacking aerial mycelium formation were compared with their genetic ancestor and another producing mutant with regard to the NAD(P)‐glycohydrolase activity during cultivation on different media. B

Relationship between macrotetrolide prod
Relationship between macrotetrolide production and specific activity of some hydrolases in a high and a low producing strain ofStreptomyces griseus
✍ Jochen Schindelmeiser; Hermann Pape 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 537 KB

The activities of five hydrolytic enzymes in the culture filtrate and in cell-free extracts from strains of Streptomyces griseus, differing in macrotetrolide production, have been determined over a fermentation period of 200 h. The specific activities of phosphatase, phosphodiesterase, and adenosine

β-1.3.-1.4-Glucanase in spore-forming mi
β-1.3.-1.4-Glucanase in spore-forming microorganisms. VI. Genetic instability of β-glucanase production in a high-producer strain of Bacillus amyloliquefaciens grown in a chemostat
✍ Dr. R. Borriss; D. Noack; R. Geuther 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 410 KB

## Abstract A __Bacillus amyloliquefaciens__ strain high‐producing for β‐1.3‐1.4‐glucanase has gradually lost the ability to produce this enzyme during long‐time continuous cultivation, independent of the culture conditions. Mutant strains isolated after long‐term cultivation exhibited changed beha