Genetic regulation of myelin basic protein expression in the mouse brain
โ Scribed by Carson, J.H.
- Book ID
- 123583855
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 72 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0736-5748
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The expression of myelin proteins in actively myelinating quaking and control brains was studied. RNA was extracted from the brains of 18- and 27-day-old mice and analyzed by northern blot using cDNA probes for proteolipid protein (PLP), basic protein (BP), and myelin-associated glycoprotein (MAG).
Polysomal poly (A)+ RNA and total cellular RNA were isolated from the brains of C57BL/6J (B6), DBA/2J (D2), and F1 hybrid mice. Both types of RNA were isolated at 9, 12, 16, 21, and 30 days of age. By using a 32P-labelled cDNA probe for myelin basic protein (MBP; pMBP-1) the amounts of RNA coding fo