𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Genetic model of transformation and neoplastic progression in laryngeal epithelium

✍ Scribed by César Álvarez Marcos; Marta Alonso-Guervós; Nuria Rodríguez Prado; Teresa Sampedro Gimeno; Francisco Domínguez Iglesias; Mario Hermsen; José Luis Llorente

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
702 KB
Volume
33
Category
Article
ISSN
1043-3074

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background

The aim of this study was to analyze genetic alterations in the transformation‐progression model of laryngeal tumors.

Methods

Copy number changes of 37 genes were analyzed by multiple ligation‐dependent probe amplification (MLPA) in 94 tissue samples.

Results

In the smoker normal mucosa group TP53 loss was predominant, whereas in the precursor lesions CDKN2A loss and CDKN2D gain were most frequent. Precursor lesions with progression presented CTNNB1 loss. In the carcinoma group the most common changes were CDKN2A, MLH1, CTNNB1, and CASP6 losses and RECQL4, CCND1, and EMS1 gains. Positive lymph node primary tumors were related to TP53, IL1A, and RB1 losses and STK11 gain. The lymph node metastases differed from their corresponding primary tumor in LMNA, RECQL4, and IGF1R losses, and N33 and CDKN2D gains.

Conclusions

Genetic changes and new key genes were found to be associated with specific steps. We included new steps, not presented in the classic models: normal mucosa tobacco exposed, positive lymph node primary tumor, and corresponding lymph node metastases. © 2010 Wiley Periodicals, Inc. Head Neck, 2011

📜 SIMILAR VOLUMES

Multiple stages and genetic alterations
Multiple stages and genetic alterations in immortalization, malignant transformation, and tumor progression of human skin keratinocytes
✍ Norbert E. Fusenig; Petra Boukamp 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 185 KB

An in vitro carcinogenesis model of human skin keratinocytes has been developed based on the spontaneously immortalized keratinocyte cell line HaCaT. Immortalization, the initial stage in human carcinogenesis in vitro, was induced by ultraviolet-type mutations in the p53 gene followed by further gen

Autonomy of the epithelial phenotype in
Autonomy of the epithelial phenotype in human ovarian surface epithelium: Changes with neoplastic progression and with a family history of ovarian cancer
✍ Helen G. Dyck; Thomas C. Hamilton; Andrew K. Godwin; Henry T. Lynch; Sarah Maine 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 French ⚖ 949 KB

Epithelial ovarian carcinomas originate in the ovarian surface epithelium (OSE). In culture, OSE undergoes epithelio-mesenchymal conversion, an event mimicking a wound response, while ovarian carcinomas retain complex epithelial characteristics. To define the onset of this increased epithelial auton

Transformation of benign Barrett's epith
Transformation of benign Barrett's epithelium by repeated acid and bile exposure over 65 weeks: A novel in vitro model
✍ Kiron M. Das; Yingxin Kong; Manisha Bajpai; Diptee Kulkarni; Xin Geng; Pravin Mi 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 French ⚖ 743 KB

## Abstract The mechanism by which gastroesophageal reflux promotes metaplasia→dysplasia→carcinoma is unknown. The aim of the study is to determine if repeated exposure to acid and bile confers a tumorigenic phenotype in a telomerase (hTERT)‐immortalized benign Barrett's cell line (BAR‐T). BAR‐T ce