Genetic Manipulation of Primate Embryonic and Hematopoietic Stem Cells with Simian Lentivirus Vectors
β Scribed by Yutaka Hanazono; Takayuki Asano; Yasuji Ueda; Keiya Ozawa
- Book ID
- 114342080
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 752 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1050-1738
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## Abstract ## Background Embryonic stem (ES) cells continually proliferate and can generate large numbers of differentiated cells. Genetic manipulation of transplantable cells derived from primate ES cells offers considerable potential for development research and regenerative cell therapy. Howev
## Abstract Summary: Mutant Ξ» integrases catalyze siteβspecific recombination reactions inside mammalian cells. Here we demonstrate that the integrase system can be used to eliminate resistance marker genes from the genome of mouse embryonic stem cells. Soβcalled integrative and excisive recombinat