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Genetic fine structure of the pyrE region containing the genes for ribosomal proteins L28 and L33 in Escherichia coli

✍ Scribed by Isono, Katsumi ;Schnier, Joachim ;Kitakawa, Madoka

Publisher: Springer
Year: 1980
Tongue: English
Weight: 593 KB
Volume: 179
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425458

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✦ Synopsis

Temperature-sensitive mutants harbouring alterations in ribosomal proteins L28 and L33 have been isolated and used in mapping the genes coding for the two proteins. It was found that they mapped very close to each other and near pyrE at 80.7 min on the E. coli genetic map. The genes affected by the mutations have been concluded to be the structural genes for proteins L28 (rpmB) and L33 (rpmG) by constructing merodiploids heterozygous for pyrE and for the two ribosomal proteins. Various transduction studies with Plkc phages indicate the gcne order in this region to be (rpmB, rpmG)-pyrE-spoT-gItC.

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