Genetic Engineering: Principles and Methods: Principles and Methods

✍ Scribed by Milton H. Saier Jr. (auth.), Jane K. Setlow (eds.)

• Publisher: Springer US
• Year: 2002
• Tongue: English
• Leaves: 241
• Series: Genetic Engineering 23
• Edition: 1
• Category: Library

✦ Synopsis

Over the past decade, our laboratory and others have been concerned with molecular archaeological studies aimed at revealing the origins and evolutionary histories of permeases (1). These studies have revealed that several different families, defined on the basis of sequence similarities, arose independently of each other, at different times in evolutionary history, following different routes. When complete microbial genomes first became available for analysis, we adapted p- existing software and designed new programs that allowed us quickly to identify probable transmembrane proteins, estimate their topologies and determine the likelihood that they function in transport (2). This work allowed us to expand previously-recognized families and to identify dozens of new families. All of this work then led us to attempt to design a rational but comprehensive classification system that would be applicable to the complete complement of transport systems found in all living organisms (3). The classification system that we have devised is based primarily on mode of transport and energy coupling mechanism, secondarily on molecular phylogeny, and lastly on the substrate specificities of the individual permeases (4).

✦ Table of Contents

Evolution of Transport Proteins....Pages 1-10
Mechanisms of Apoptosis Repression....Pages 11-33
Cytokine Activation of Transcription....Pages 35-44
Enzymatic Approaches to Glycoprotein Synthesis....Pages 45-68
Vector Design and Development of Host Systems for Pseudomonas ....Pages 69-81
Genetic and Biochemical Studies on the Assembly of an Enveloped Virus....Pages 83-112
Enzyme and Pathway Engineering for Suicide Gene Therapy....Pages 113-127
Deconstructing a Conserved Protein Family: The Role of MCM Proteins in Eukaryotic DNA Replication....Pages 129-155
Expression of Foreign Genes in the yeast Pichia pastoris ....Pages 157-169
Protein Splicing and its Applications....Pages 171-199
Global Transcript Expression Profiling by Serial Analysis of Gene Expression (SAGE)....Pages 201-219

✦ Subjects

Human Genetics; Biochemistry, general; Plant Sciences