Genetic effects of petroleum fuels: II. Analysis of chromosome loss and hyperploidy in peripheral lymphocytes of gasoline station attendants
✍ Scribed by A. Carere; A. Antoccia; D. Cimini; R. Crebelli; F. Degrassi; P. Leopardi; F. Marcon; A. Sgura; C. Tanzarella; A. Zijno
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 337 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
Molecular cytogenetic methods were applied to in-
The results obtained did not show any exposurevestigate the effect of the occupational exposure to related excess of hyperploidy or micronucleus forlow concentrations of benzene and petroleum fuels mation. Conversely, the age of the subjects was on genomic stability. Twelve male gasoline station significantly correlated with several markers of geattendants (average benzene exposure of 0.32 nomic instability, such as the incidence of chromomg/m 3 as 8h TWA) and 12 age-and smoking-some X and chromosome 18 hyperploidy, total hymatched unexposed controls were selected for the perploidy and polyploidy, and close to statistical study. The incidence of hyperploidy and polyploidy significance with chromosome loss. Smoking habits in peripheral lymphocytes was evaluated through did not appear to contribute significantly to the efin situ hybridization of interphase cells, harvested fects measured. 24 hr after stimulation, with centromeric probes
The parallel analysis of hyperploidy and polyof chromosomes 7, 11, 18, and X. For half of the ploidy in interphase nuclei in 24-hr cultures and in subjects, metaphases harvested 24 hr later were metaphase cells harvested 24 hr later showed basianalyzed. The incidence of chromosome loss in cally similar incidences of aneuploid cells, indicating vitro was determined in cytokinesis-blocked cells, that no significant selection against hyperploid and harvested at 66 hr, through the hybridization of polyploid types occurred during the first cell cycle in micronuclei with a pancentromeric probe. Ten thou-vitro. Environ. Mol. Mutagen. 32:130-138, 1998 sand chromosomes (more than 200 metaphases ᭧ 1998 Wiley-Liss, Inc. equivalent) and 2,000 binucleated cells/person were scored for hyperploidy and micronucleus analysis, respectively.