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Genetic diversity of Y-specific STRs in chimpanzees (Pan troglodytes)

✍ Scribed by L. Gusmão; A. González-Neira; C. Alves; P. Sánchez-Diz; E.M. Dauber; A. Amorim; A. Carracedo


Book ID
101450640
Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
63 KB
Volume
57
Category
Article
ISSN
0275-2565

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✦ Synopsis


Abstract

Using the primers described for humans, sequences for 11 Y‐specific microsatellites (DYS434, DYS435, DYS436, DYS437, DYS438, DYS439, GATA A10, A7.1, A7.2, C4, and H4 [Gusmão et al., in press]), previously described in 10 male chimpanzees (Pan troglodytes), were confirmed in nine additional male chimpanzees. Sequences for nine additional microsatellites (DYS19, DYS385I and II, DYS389I and II, DYS390, DYS391, DYS392, and DYS393) were determined in all 19 male chimpanzees; homology to human Y‐Short Tandem Repeat (STRs) was confirmed by sequencing. Good amplification results were not obtained for DYS19 and DYS385I/II. Two amplicons were obtained for DYS389I/II, but in contrast to humans, the larger fragment was not Y‐specific. Moreover, no polymorphism was observed for DYS434, DYS435, or GATA A10. Consequently, these eight STRs were eliminated from further analyses, and haplotype and allele frequencies were estimated for the remaining 12 STRs. A high haplotype diversity value was found (1.000 ± 0.017), demonstrating the usefulness and informative power of these Y‐STRs for future studies on chimpanzee population genetics.Am. J. Primatol. 57:21–29, 2002. © 2002 Wiley‐Liss, Inc.


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