Genetic characterization of an α-specific gene responsible for sexual agglutinability inSaccharomyces cerevisiae: mapping and gene dose effect

✍ Scribed by Katsunori Suzuki; Naohiko Yanagishima

• Publisher: Springer-Verlag
• Year: 1986
• Tongue: English
• Weight: 446 KB
• Volume: 10
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00418406

✦ Synopsis

A recessive agal mutation leads to specific defect in sexual agglutinability specifically in a cells of the yeast Saccharomyces cerevisiae. The cryptopleurine resistance gene cryr~l, closely linked to the mating type locus, was used to select a/a strains which emerged from a/a strains by mitotic nonreciprocal recombination, to genetically analyse ago.d, since agod is expressed only in a mating type. The agal gene was found to be linked to the centromere tightly, to met3 at 4.4 cM, and to ilv3 at 12 cM on chromosome X. Sexual agglutinabllity of a cells was shown to be dependent on the dose of the AGod gene, using a/a isogertic strains carrying AG~I/ AGo_l, AGal /agal or agod /agod. The sst2-1 mutation did not suppress the agal mutation. Based on these results, function of the AGod gene is discussed.