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Genetic characterization of adenovirus strains isolated from patients with acute conjunctivitis in the city of S�o Paulo, Brazil

✍ Scribed by Tanaka, Keico; Itoh, Norihiko; Saitoh-Inagawa, Waka; Uchio, Eiichi; Takeuchi, Satoshi; Aoki, Koki; Soriano, Eduardo; Nishi, Mauro; J�nior, Rubens Belfort; H�rsi, Charlotte M.; Tsuzuki-Wang, Liping; Durigon, Edison L.; Stewien, Klaus E.; Ohno, Shigeaki


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
581 KB
Volume
61
Category
Article
ISSN
0146-6615

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✦ Synopsis


Genome analysis was carried out on adenovirus strains isolated from patients with acute follicular conjunctivitis in the city of Sa ˜o Paulo, Brazil. Eighteen conjunctival scrapings, collected between December 1993 and March 1994, were analyzed by two methods: a combination of polymerase chain reaction with restriction fragment length polymorphism and viral DNA restriction analysis, carried out using 10 restriction endonucleases: BamHI, BglI, BglII, HindIII, KpnI, SacI, SalI, SmaI, XbaI, and XhoI. Among 11 adenovirus detected by cell culture isolation, nine were Ad8, and two were Ad7. By restriction analysis the Ad8 isolates were typed as two new variants-Ad8/D11 (seven of nine samples) and Ad8/D12 (two of nine samples). Ad7 isolates were identified as a subtype of the widespread genome type Ad7b and the virulent type Ad7h, a predominant genome type circulating in Argentina, Chile, and Uruguay but absent in Brazil until 1991.


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