Genetic analysis of the bchC and bchA genes of Rhodobacter sphaeroides

✍ Scribed by McGlynn, Peter ;Neil Hunter, C.

• Book ID: 104699610
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 908 KB
• Volume: 236-236
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00277117

✦ Synopsis

This study has identified by sequence analysis a single gene in the bchC locus of Rhodobacter sphaeroides and three genes, designated bchX, Y and Z, in the bchA locus, which was previously thought to contain only a single gene. All four genes may reside within the same operon and are transcribed in the order bchC-X-Y-Z. Complementation analysis of eight transposon insertion mutants within these genes suggests that bchX, Y and Z are essential for the reduction of 2-devinyl-2-hydroxyethyl chlorophyllide a and that bchC encodes the 2-desacetyl-2-hydroxyethyl bacteriochlorophyllide a dehydrogenase. Similarity between the putative BchX protein and dinitrogenase reductase proteins suggests that BchX may also be a reductase, supplying electrons for reduction of 2-devinyl-2-hydroxyethyl chlorophyllide a.