Genetic Analysis and Characterization of Poly(aspartic acid) Hydrolase-1 from Sphingomonas sp. KT-1
✍ Scribed by Hiraishi, Tomohiro; Kajiyama, Mariko; Tabata, Kenji; Yamato, Ichiro; Doi, Yoshiharu
- Book ID
- 126398454
- Publisher
- American Chemical Society
- Year
- 2003
- Tongue
- English
- Weight
- 194 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1525-7797
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## Abstract **Summary:** The enzymatic hydrolysis of __α__‐ and __β__‐oligo(L‐aspartic acid)s by PAA hydrolase‐1 and PAA hydrolase‐2 (purified from __Sphingomonas__ sp. KT‐1) was performed to elucidate the mechanism of the microbial degradation by __Sphingomonas__ sp. KT‐1 of the thermally synthesi
## Abstract __Pedobacter__ sp. KP‐2 can degrade and metabolize thermally synthesized __α__,__β__‐poly(D,L‐aspartic acid) (tPAA), which contains 70% of unnatural __β__‐amide units, with high‐molecular‐weight. In this study, gene cloning and molecular characterization of PAA hydrolase‐1 from KP‐2 was