We studied the rDNA spacer length polymorphism in a sample of 121 individuals belonging to families of 2-3 generations. Our data, obtained by restriction pattern analysis of genomic DNA, confirmed the limited and discrete nature of this polymorphism. Using the pattern as a genetic marker, we analyze
Genetic analyses of rDNA spacer-length variation in barley
โ Scribed by K. M. Soliman
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 705 KB
- Volume
- 85-85
- Category
- Article
- ISSN
- 0040-5752
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โฆ Synopsis
The genetic mechanism controlling the inheritance of single and multiple spacer-length variant (slv) phenotypes in barley was investigated in six F2 segregating populations. The results indicated that two independently assorting loci, each with co-dominant alleles, govern genetic variability for rDNA in barley regardless of the number of bands expressed by a given phenotype. The following chromosomal locations are proposed: sl variants 1, 4, 5, 6, and 7 on chromosome 7, and sl variants 7, 8, 9, 12, and 13 on chromosome 6; sl variant 7 is thus located on both of the chromosomes.
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The structure and variation of nuclear ribosomal DNA (rDNA) units of Picea abies, (L.) Karst. was studied by restriction mapping and Southern hybridization. Conspicuous length variation was found in the internal transcribed spacer (ITS) region of P. abies, although the length of this region is highl
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