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Genes of succinyl-CoA ligase from Saccharomyces cerevisiae

✍ Scribed by Beata Przybyla-Zawislak; Richard A. Dennis; Stanislav O. Zakharkin; Mark T. McCammon


Book ID
114429531
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
408 KB
Volume
258
Category
Article
ISSN
1432-1327

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Succinyl-CoA synthetase from Saccharomyces cerevisiae was partially purified (20-fold) with a yield of 44%. The Michaelis-Menten constants were determined: Km (succinate) = 17 mM; Km (ATP) = 0.13 mM; Km (CoA) = 0.03 mM. The succinyl-CoA synthetase has a molecular weight of about 80000 dalton (as det

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