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Genes for ribosomal proteins are retained on the 73 kb DNA fromAstasia longathat resemblesEuglenachloroplast DNA

✍ Scribed by Gerhard Siemeister; Christiane Buchholz; Wolfgang Hachtel

Publisher: Springer-Verlag
Year: 1990
Tongue: English
Weight: 748 KB
Volume: 18
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00309917

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✦ Synopsis

The nucleotide sequence of a 6.7 kb segment of the circular 73 kb DNA from Astasia longa has been determined. We identified genes for a tRNA-Ile (CAU), a tRNA-Phe (GAA), a tRNA-Cys (GCA) and the ribosomal proteins CS8, CL36, CS14 and CS2, that are normally encoded by plastid genomes. In addition, a gene for the chloroplast ribosomal protein CL5 was found that is not encoded by the plastome in either higher plants or a liverwort, but has recently been identified in Euglena chloroplast DNA. Transcripts of these protein genes, and of an unidentified open reading frame (ORF50), were detected. These results support our previous suggestion that the 73 kb DNA from Astasia is a truncated form of plastid DNA. The 73 kb DNA resembles the chloroplast DNA of Euglena gracilis but contains, almost exclusively, genes for a plastid-type translational (and presumably transcriptional) apparatus.

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Genes for the plastid elongation factor Tu and ribosomal protein S7 and six tRNA genes on the 73 kb DNA from Astasia longa that resembles the chloroplast DNA of Euglena

✍ Siemeister, Gerhard ;Buchholz, Christiane ;Hachtel, Wolfgang 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 813 KB

The nucleotide sequence of a 6156 bp segment of the circular 73 kb DNA from Astasia longa resembling the chloroplast DNA of Euglena was determined. The genes for the plastid elongation factor Tu (tufA) and the ribosomal protein $7 (rps7), six tRNA genes (trnQ, trnS, trnG, trnM, trnT, trnR), and thre