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Generation of various amino acids mutants in the trpR gene of Escherichia coli by site-directed mutagenesis

✍ Scribed by C.R. Marutha Ravindran


Book ID
116750300
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
331 KB
Volume
70
Category
Article
ISSN
0167-7012

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The expression of the trpR gene of Escherichia coli was investigated by measuring trpR messenger RNA levels in vivo under various physiological conditions. Trp repressor, when present, led to significant decreases in the amount of trpR message produced; this effect was enhanced by providing excess L