## Abstract ## Background Short hairpin RNA (shRNA) libraries are considered to comprise a powerful tool in genetic screening. Several library construction methods have been proposed; these methods require between four and nine tedious reaction steps to construct the library. In the present study,
Generation of shRNA Pool Library: A Revision of the Biological Technique from the Viewpoint of Chemistry
β Scribed by Demin Zhou; Cuiying Wang; Jing Zhang; Josh Bliesath; Qiuchen S. He; Ning Ke; Dehua Yu; Qixiang Li; Li-He Zhang; Flossie Wong-Staal
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 285 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1439-4227
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Using Stiefel manifolds (real, complex, and symplectic) as examples, it is shown that (A) 5 (B) n (C) $ (C) in a differentiable sense, where (A) denotes the class of all regular Riemannian &symmetric spaces (k 2 2), (B) th e c ass of all pointwise Riemannian k-symmetric 1 spaces (L 2 2), and (C) the
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A polymerase chain reaction (PCR)-based technique is described which allows for the determination of library plasmid insert DNA sequence directly and rapidly from intact yeast cells. Yeast spheroplasts are used to template a PCR reaction to amplify the insert sequence. This PCR product is then purif