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Generation of Saccharomyces cerevisiae deletants and basic phenotypic analysis of eight novel genes from the left arm of chromosome XIV

✍ Scribed by M. Maftahi; C. Gaillardin; J.-M. Nicaud


Book ID
119982757
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
195 KB
Volume
14
Category
Article
ISSN
0749-503X

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We describe here the construction of six deletion mutants and their basic phenotypic analysis in three different backgrounds. The six genes were disrupted in three diploid strains (FY1679, W303 and CEN.PK2) by the long flanking homology (LFH) method (Wach, 1996). Transformants were selected as genet

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Six open reading frames (ORFs) of unknown function from the left arm of Saccharomyces cerevisiae chromosome XV were deleted in two genetic backgrounds by disruption cassettes with long flanking homology (LFH) , within the frame of the research project EUROFAN. The LFH disruption cassettes, obtained