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Generation of Mammalian Cells Stably Expressing Multiple Genes at Predetermined Levels

โœ Scribed by Xuedong Liu; Stefan N. Constantinescu; Yin Sun; Jonathan S. Bogan; David Hirsch; Robert A. Weinberg; Harvey F. Lodish

Book ID
102561962
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
280 KB
Volume
280
Category
Article
ISSN
0003-2697

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## Abstract A set of mutated SV40 early polyadenylation signals (SV40pA) with varying strengths is generated by mutating the AATAAA sequence in the wildโ€type SV40pA. They are shown to control the expression level of a gene over a 10โ€fold range using luciferase reporter genes in transient transfecti