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Generation of long-lived B cells in germ-free mice

✍ Scribed by Irmgard Förster; Werner Müller; Birgit Schittek; Klaus Rajewsky


Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
361 KB
Volume
21
Category
Article
ISSN
0014-2980

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## Abstract Summary: Two recombination steps in embryonic stem (ES) cells were adopted to generate a floxed __Germ Cell Nuclear Factor__ (__GCNF__) allele. First, a targeting vector containing a loxP site upstream of exon 4, encoding the DNA binding domain (DBD), and a floxed __NeoTK__ double selec