Generation of a germ cell nuclear factor
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Zi-Jian Lan; Xueping Xu; Austin J. Cooney
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Article
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2003
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John Wiley and Sons
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English
⚖ 495 KB
👁 1 views
## Abstract Summary: Two recombination steps in embryonic stem (ES) cells were adopted to generate a floxed __Germ Cell Nuclear Factor__ (__GCNF__) allele. First, a targeting vector containing a loxP site upstream of exon 4, encoding the DNA binding domain (DBD), and a floxed __NeoTK__ double selec