Generation of hydrogen peroxide by human neutrophils: Effects of soluble stimuli and requirements for divalent cations
โ Scribed by Robert J. Smith; Barbara J. Bowman
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 79 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0192-0561
No coin nor oath required. For personal study only.
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The chemiluminescence (CL) technique with scavengers for superoxide anion (superoxide dismutase) and hydrogen peroxide (catalase) was used to characterize the generation of reactive oxygen species (ROS) inside and outside the human neutrophil after stimulation with both soluble (formyl-methionyl-leu
## ABSTRACT Human neutrophils suspended in Hanks' balanced salt solution (37ยฐ C, 20 mM Hepes, pH 7.2) produced extensions, elongated and developed a polarised morphology with both a pseudopod and uropod when exposed to C5a (10 nM), leukotriene B4 (10 nM), platelet activating factor (40 nM) or inter