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Generation of cloned calves and transgenic chimeric embryos from bovine embryonic stem-like cells

โœ Scribed by Shigeo Saito; Ken Sawai; Hideyo Ugai; Satoru Moriyasu; Akira Minamihashi; Yusuke Yamamoto; Hiroki Hirayama; Soichi Kageyama; Jianzhi Pan; Takehide Murata; Yoshiro Kobayashi; Yuichi Obata; Kazunari K Yokoyama


Book ID
117054524
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
206 KB
Volume
309
Category
Article
ISSN
0006-291X

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Mice chimeric for embryonic stem (ES) cells have not always successfully produced ES-derived offspring. Here we show that the male gametes from ES cells could be selected in male chimeric mice testes by labeling donor ES cells or host blastocytes with GFP. Male GFP-expressing ES-derived germ cells o