Generation of cDNA Libraries. Methods and Protocols
โ Scribed by Shao-Yao Ying
- Publisher
- Humana Press
- Year
- 2010
- Tongue
- English
- Leaves
- 331
- Series
- Methods in Molecular Biology
- Edition
- 1st Edition.
- Category
- Library
No coin nor oath required. For personal study only.
โฆ Synopsis
Expert researchers and inventors in the field describe their own proven techniques for generating cDNA/mRNA libraries to identify the functions of specific decoded gene sequences. A wide variety of techniques is presented for enhancing the generation of complete and full-length libraries, and for confirming the quality of the cDNAs generated. Among the applications detailed are electrophoresis, Northern blotting, single cell microarray analysis, subtractive hybridization, subtractive cloning, gene cloning, and peptide library generation.
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<p>With the decoding of the human genome and the potential application of its information to gene chips, the ability to generate cDNA libraries for functional assays of specific gene sequences has become critical in gene manipulation and genetic research. In Generation of cDNA Libraries: Methods and
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This comprehensive collection of detailed protocols covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widely used protocols with some very useful newer