Generation of CD4+ cytotoxic T lymphocytes stimulated by immobilized anti-CD3 monoclonal antibody and interleukin-2 in cancer patients

✍ Scribed by Masaji Tani; Hiroshi Tammura; Hiroki Yamaue; Shizuma Mizobata; Makoto Iwahashi; Takuya Tsunoda; Kohci Noguchi; Mikiko Tamai; Tsukasa Hotta; Hiroshi Terasawa; Kazuo Arii

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: French
• Weight: 645 KB
• Volume: 60
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910600613

✦ Synopsis

The proliferation of autologous tumor-reactive cytotoxic T lymphocytes (CTL), induced by autologous mixed lymphocyte tumor-cell culture, was remarkably enhanced by activation with immobilized anti-CD3 monoclonal antibody (MAb) and interleukin-2 (IL-2), as compared with IL-2 alone. The activated CTL exhibited high cytotoxicity against autologous tumor cells. Cytotoxicity against autologous tumor cells was inhibited by anti-HIA-DR MAb. In negative selection with immunomagnetic beads, cytotoxicity against autologous tumor cells was inhibited by the elimination of CD4+ cells. The major cell-surface antigens of the activated CTL were CD3+, CD4+, CD25+, CD45RO+ and CD45RA-, suggesting helper T cells, and the activated CTL produced IL-2. It is concluded that the CTL activated by immobilized anti-CD3 MAb and IL-2 were CD4 cells that had both killer and helper functions. Our findings indicate that adoptive immunotherapy using these activated CTL would be effective in cancer patients.