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Generation of an Electrochemical Proton Gradient by Lactate Efflux in Membrane Vesicles of Escherichia coli

โœ Scribed by Bart TEN BRINK; Wil N. KONINGS


Book ID
115119416
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
761 KB
Volume
111
Category
Article
ISSN
1432-1327

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A rapid and simple method for separating and isolating the inner and outer membranes of Escherichia coli is described. Membrane vesicles were prepared either by passing the bacteria through a French press or by conversion of the cells to spheroplasts by the lysozyme-EDTA treatment and disruption of