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Generation of a fluorescently labeled endogenous protein library in living human cells

โœ Scribed by Sigal, Alex; Danon, Tamar; Cohen, Ariel; Milo, Ron; Geva-Zatorsky, Naama; Lustig, Gila; Liron, Yuvalal; Alon, Uri; Perzov, Natalie

Book ID
109946188
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
513 KB
Volume
2
Category
Article
ISSN
1750-2799

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โœ Renรฉ Michel; Ralf Steinmeyer; Michael Falk; Gregory S. Harms ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 652 KB

## Abstract A new algorithm is presented for the detection of single, fluorescenceโ€labeled proteins in the analysis of images from living cells. It is especially suited for images with just a few (<1 per 10 ฮผm^2^) fluorescence peaks from individual proteins with high background and noise (signal to