Generation of a conditional allele of the B-myb gene

✍ Scribed by Paloma García; Oscar Berlanga; Roger Watson; Jon Frampton

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 630 KB
• Volume: 43
• Category: Article
• ISSN: 1526-954X
• DOI: 10.1002/gene.20170

✦ Synopsis

Abstract

B‐Myb is an essential transcription factor involved in control of the cell cycle and the regulation of tissue‐specific gene expression in a wide range of cell types. Loss of both alleles results in early embryonic lethality at E4.5–6.5. To address the function of B‐Myb in later stages of embryogenesis and in specific adult tissues, a floxed B‐myb allele (B‐mybF) was generated. Cre‐mediated deletion in vivo was demonstrated by breeding with a transgenic GATA‐Cre mouse line. An intermediate allele produced in the creation of the floxed allele, in which the PGK‐neo^R^ cassette is present in intron 3 (B‐myb^loxneo^), was deduced to be a weak hypomorph based on the later embryonic death of homozygotes compared to B‐myb^−/−^ embryos. To demonstrate the efficiency and possible consequences of B‐myb inactivation, we performed conditional deletion in cultured MEFs and observed decreased growth that correlated with aberrant nuclear DNA replication. genesis 43:189–195, 2005. © 2005 Wiley‐Liss, Inc.