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Generation of a cholesterol-independent, non-GS NS0 cell line through chemical treatment and application for high titer antibody production

✍ Scribed by Jincai Li; Wei Gu; Diane G. Edmondson; Connie Lu; Natarajan Vijayasankaran; Bruno Figueroa; Dave Stevenson; Thomas Ryll; Feng Li

Book ID
112012809
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
362 KB
Volume
109
Category
Article
ISSN
0006-3592

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## Abstract Presented is an antibody production platform based on the fed‐batch culture of recombinant NS0‐derived cell lines. NS0 host cells, obtained from the European Collection of Cell Cultures (ECACC, Salisbury, UK, Part No. 85110503), were first adapted to grow in a protein‐free, cholesterol‐