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Generation and application of tools for increasing periplasmic soluble product yields of recombinant therapeutic proteins in Escherichia coli

✍ Scribed by Büttner, Alexander; Stammen, Simon; Dürkop, Mark; Schuller, Artur; Heistinger, Lina

Book ID
126467535
Publisher
Elsevier
Year
2014
Tongue
English
Weight
60 KB
Volume
31
Category
Article
ISSN
1871-6784

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## Abstract Many enzymes or fluorescent proteins produced in __Escherichia coli__ are enzymatically active or fluorescent respectively when deposited as inclusion bodies. The occurrence of insoluble but functional protein species with native‐like secondary structure indicates that solubility and co