General Method for the Determination of Stability Constants of Lanthanide Ion Chelates by Ligand−Ligand Competition: Laser-Excited Eu 3+ Luminescence Excitation Spectroscopy

The determination of the Mg2+·ATP dissoc

The determination of the Mg2+·ATP dissociation constant by competition with Eu3+ ion using laser-induced Eu3+ ion luminescence spectroscopy

✍ Charles W. McNemar; William DeW. Horrocks Jr. 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 419 KB

A direct spectroscopic method for the determination of the submicromolar dissociation constant of Eu3'. ATP using laser-induced Eu3+ ion luminescence spectroscopy is described. The dissociation constant of Mg2+ .ATP is then determined by the competition of Mg2+ with Eu3+ for the binding of ATP. The