Bacterial -galactosidase cDNA was injected without lipofectin into 41 sites in dog muscle and expression was seen in 22 of them. The cDNA and lipofectin was injected into 35 similar sites and expression was seen in 21. Expression was seen in a maximum of 2.5% of muscle fibers and 23.21% of nonmuscl
Gene transfer into individual muscle fibers and conditional gene expression in living animals
✍ Scribed by Andreas Sander; Antje Güth; Hans Rudolf Brenner; Veit Witzemann
- Publisher
- Springer-Verlag
- Year
- 2000
- Tongue
- English
- Weight
- 122 KB
- Volume
- 301
- Category
- Article
- ISSN
- 0302-766X
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## Abstract ## Background Degenerative or traumatic tendon injuries are extremely common but often heal poorly, not restoring the normal function of the injured tissues. Gene transfer could improve the repair process, by permitting local production of therapeutic substances, e.g. growth factors.
The ability to monitor reporter gene expression in living animals and in patients will permit longitudinal examinations both of somatically transferred DNA in experimental animals and patients and of transgenic constructs expressed in experimental animals. If investigators can non-invasively monitor