𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Gene transfer into guinea pig cochlea using adeno-associated virus vectors

✍ Scribed by Masaya Konishi; Kohei Kawamoto; Masahiko Izumikawa; Hiromichi Kuriyama; Toshio Yamashita

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
384 KB
Volume
10
Category
Article
ISSN
1099-498X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background

Several genes are candidates for treating inner ear diseases. For clinical applications, minimally invasive approaches to the inner ear are desirable along with minimal side‐effects.

Methods

Adeno‐associated virus (AAV) was used as a vector into the guinea pig inner ear. Six AAV‐cytomegalovirus hybrids (AAV‐2/1, ‐2/2, ‐2/5, ‐2/7, ‐2/8 and ‐2/9) were infused into perilymph of the cochlea basal turn, an approach that could be used in cochlear implant surgery. At 7 days after injection, distribution of gene expression, hearing and morphology were evaluated. Adenoviral vector was also used to compare distributions of gene expression. Moreover, distribution of cell surface receptors of AAV in the cochlea was examined using immunohistochemistry.

Results

Using the perilymphatic approach, adenovirus could be transferred to mesothelial cells lining the perilymph, but not sensory cells. Conversely, all AAV serotypes displayed tissue tropism to inner hair cells, with AAV‐2/2 showing particularly efficient transfer to sensory cells. This tissue tropism of AAV could not be explained by the distribution of AAV receptors. Hearing and morphology were largely unaffected.

Conclusions

Our results indicate that AAV vector can be safely applied to the inner ear and AAV‐2/2 offers a good tool for transferring transgenes into sensory cells of the inner ear efficiently without toxicity. Copyright © 2008 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Gene transfer into rabbit arteries with
Gene transfer into rabbit arteries with adeno-associated virus and adenovirus vectors
✍ Marcin Gruchała; Shalini Bhardwaj; Katri Pajusola; Himadri Roy; Tuomas T. Rissan 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 352 KB

## Abstract ## Background Gene transfer offers considerable potential for altering vessel wall physiology and intervention in vascular disease. Therefore, there is great interest in developing optimal strategies and vectors for efficient, targeted gene delivery into a vessel wall. ## Methods We

Transient expression of genes delivered
Transient expression of genes delivered to newborn rat liver using recombinant adeno-associated virus 2/8 vectors
✍ Maude Flageul; Dominique Aubert; Virginie Pichard; Tuan Huy Nguyen; Ali Nowrouzi 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 217 KB

## Abstract ## Background __In vivo__ adeno‐associated virus (AAV) delivery to adult liver results in sustained expression of the transgene. However, it has been suggested that AAV delivery to the newborn liver may result in transient expression. In the present study, we analysed transgene express

Correction of mutant Fanconi anemia gene
Correction of mutant Fanconi anemia gene by homologous recombination in human hematopoietic cells using adeno-associated virus vector
✍ Kittiphong Paiboonsukwong; Fumi Ohbayashi; Haruka Shiiba; Emi Aizawa; Takayuki Y 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 288 KB

## Abstract ## Background Adeno‐associated virus (AAV) vectors have been shown to correct a variety of mutations in human cells by homologous recombination (HR) at high rates, which can overcome insertional mutagenesis and transgene silencing, two of the major hurdles in conventional gene addition

Specific adeno-associated virus serotype
Specific adeno-associated virus serotypes facilitate efficient gene transfer into human and non-human primate mesenchymal stromal cells
✍ Keefe Chng; Stephen R. Larsen; Shangzhen Zhou; J. Fraser Wright; Rosetta Martini 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 398 KB

## Abstract Mesenchymal stromal cells (MSCs) show great promise for __ex vivo__ gene and cell‐mediated therapies. The immunophenotype and __in vitro__ differentiation capacity of primary baboon MSCs was demonstrated to be near‐identical to that observed in human MSCs. To optimize gene transfer effi

Efficient production of Fah-null heteroz
Efficient production of Fah-null heterozygote pigs by chimeric adeno-associated virus-mediated gene knockout and somatic cell nuclear transfer
✍ Raymond D. Hickey; Joseph B. Lillegard; James E. Fisher; Travis J. McKenzie; Sea 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 466 KB

Hereditary tyrosinemia type I (HT1) results in hepatic failure, cirrhosis, and hepatocellular carcinoma (HCC) early in childhood and is caused by a deficiency in the enzyme fumarylacetoacetate hydrolase (FAH). In a novel approach we used the chimeric adeno-associated virus DJ serotype (AAV-DJ) and h

Detectable reporter gene expression foll
Detectable reporter gene expression following transduction of adenovirus and adeno-associated virus serotype 2 vectors within full-thickness osteoarthritic and unaffected canine cartilage in vitro and unaffected guinea pig cartilage in vivo
✍ Kelly S. Santangelo; Sarah A. Baker; Gerard Nuovo; Jonathan Dyce; Jeffrey S. Bar 📂 Article 📅 2009 🏛 Elsevier Science 🌐 English ⚖ 286 KB

## Abstract This study quantified and compared the transduction efficiencies of adenoviral (Ad), Arg‐Gly‐Asp (RGD)‐modified Ad, adeno‐associated viral serotype 2 (AAV2), and self‐complementary AAV2 (scAAV2) vectors within full‐thickness osteoarthritic (OA) and unaffected canine cartilage explants i