Gene therapy: Adenovirus-mediated human bone morphogenetic protein-2 gene transfer induces mesenchymal progenitor cell proliferation and differentiation in vitro and bone formation in vivo
✍ Scribed by Jueren Lou; Fang Xu; Kurt Merkel; Paul Manske
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 847 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0736-0266
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✦ Synopsis
This study rcports that recombinant adenovirus-mediated human bone morphogenetic protein-2 gene transfer can induce mesenchymal progenitor cell dillerentialion and bone formation. The recombinant adenovirus with the human hone morphogenetic protein-2 gene was constructed, and mature human bone morphogenetic protein-2 expression mediated by adenovirus gene transler was detectcd by specific antibody. Under adenovirus-mediated bone morphogenetic-protein gene transfer, mesenchymal progenitor cell line C.?H/lOT 112 showed cell proliferation dependent on adenovirus bone morphogenetic-protein dose. The C3HIlOT 112 cells transduced by adenovirus bone morphogenetic protein also exhibited differentiation t o osteoblast phenotype, which indicates alkaline phosphatase activity. Injection of the C3HIlOT 1/2 cells into the thigh muscles of nude mice led t o ossicle development detectable on radiographs. Histological analysis indicated that the new ossicles that developed in the thigh muscles of the mice had different osseous components including bone trabeculae, bone marrow. and chondrified tissue. The results of this study demonstrate the potcntial for gene therapy by adenovirus-mediated bone morphogenetic-protein gene transfer.