𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Gene silencing in mammalian cells by uptake of 5-methyl deoxycytidine-5′-triphosphate

✍ Scribed by Robin Holliday; Thu Ho

Book ID
112501325
Publisher
Springer
Year
1991
Tongue
English
Weight
616 KB
Volume
17
Category
Article
ISSN
1572-9931

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Palindromic Oligonucleotide-Directed Enz
Palindromic Oligonucleotide-Directed Enzymatic Determination of 2′-Deoxythymidine 5′-Triphosphate and 2′-Deoxycytidine 5′-Triphosphate in Human Cells
✍ Hao Zhang; Owen L. Wood; Susan F. Papermaster; Carl J. Nielsen; Michael A. Usser 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 177 KB

A new method is presented for the determination of 2'-deoxythymidine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate concentrations within human cells based on a DNA polymerase reaction directed by a palindromic oligonucleotide precursor. Two 19-mer oligonucleotide precursors are employed that

The quantitative determination of 2′-deo
The quantitative determination of 2′-deoxycytidine-5′-triphosphate in cell extracts by radioimmunoassay
✍ Evelyn M. Piall; G.Wynne Aherne; Vincent Marks 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 459 KB

A radioimmunoassay (RIA) capable of quantitating dCTP in femtomolar amounts in cell extracts has been developed, and applied to human fibroblast cell lines and L5178Y mouse lymphoma lines. Cross reactivity of the antibody with CTP, though low (2.7%) has necessitated pre-RIA removal of CTP by either