Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli
β Scribed by Christopher D Herring; Jeremy D Glasner; Frederick R Blattner
- Book ID
- 117521340
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 739 KB
- Volume
- 311
- Category
- Article
- ISSN
- 0378-1119
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A method to obtain amber mutations in ribosomal protein genes is described. tit relies on the P1-mediated localized mutagenesis (Hong and Ames, 1971) and on the fact that the recipient strain contains (a) an efficient but genetically unstable suppressor, (b) a particular thermoinducible lambda proph
Amber mutants of Escherichia coli K-12 affected in the structural gene (rpoD) for th a subunit ofRNA polymerase have been obtained from a strain harboring a temperature-sensitive amber suppressor (supF-Ts6) which is active only at low temperatures. These mutants grow normally at low temperature (30
The dasC mutation, an extragenic suppressor of dnaA46, was mapped by P1 transduction near the rep, trxA, rho region of the Escherichia coli chromosome. The dasC mutation could not be separated from trxA by P1 transduction indicating that dasC and trxA are allelic. Multicopy plasmids containing an in