Gene replacement and ectopic integration in the zygomyceteMucor circinelloides

✍ Scribed by José Arnau; Per Strøman

• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 631 KB
• Volume: 23
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00312649

✦ Synopsis

Transformation of Mucor circinelloides with plasmid pTL42 led to the identification of different integrative events. Analysis of eight transformed strains allowed the detection of three classes of transformants: integrative types derived from either homologous (exclusively at the leuA locus) or heterologous recombination and a class which originated by gene replacement. No autonomous replication of the vector was observed.