๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Gene mutation assay of acrolein in the CHO/HGPRT test system

โœ Scribed by Richard A. Parent; Halina E. Caravello; John W. Harbell

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
513 KB
Volume
11
Category
Article
ISSN
0260-437X

No coin nor oath required. For personal study only.

โœฆ Synopsis

The mutagenic potential of acrolein has been studied with a wide range of in vitro and in vivo genetic toxicity assays. The data often have been conflicting, especially with the Ames assay. This study was undertaken to assess the mutagenic potential of acrolein using the CHOlHGPRT assay, both with and without metabolic activation. This assay system was chosen because it provides eukaryotic DNA as the target and is capable of detecting a range of mutational events. Because of its considerable toxicity, acrolein was tested over a very narrow dose range of 0.2-2 nl m l -' without exogenous activation and 0.5-8 nl m1-I with rat S-9 activation. Multiple assays were performed under both conditions. The results indicated that while acrolein was clearly very cytotoxic, it did not induce a significant mutagenic response in the presence or absence of metabolic activation.

๐Ÿ“œ SIMILAR VOLUMES

Evaluation of the division arrest method
Evaluation of the division arrest method of the CHO/HGPRT mutation assay
โœ Y. Oshiro; P. S. Balwierz; C. E. Piper ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 390 KB

We have evaluated the division arrest method of the CHO/HGPRT mutation assay following the procedure described by O'Neill et al. Environ. Mutagen. 4, 421-434 (1982). This method simplifies the culture manipulations required during the expression period and can be readily adopted for screening mutage

Evaluation of the genotoxic potential of
Evaluation of the genotoxic potential of zinc pyrithione in the Salmonella mutagenicity (Ames) assay, CHO/HGPRT gene mutation assay and mouse micronucleus assay
โœ N. P. Skoulis; S. J. Barbee; D. Jacobson-Kram; D. L. Putman; R. H. C. San ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 639 KB

## Abstract The mutagenic potential of zinc pyrithione (Znpt) was evaluated __in vitro__ in the __Salmonella__/mammalian microsome plate incorporation mutagenicity (Ames) assay and the CHO/HGPRT gene mutation assay. The clastogenic potential of Znpt was evaluated __in vivo__ using the mouse micronu

Cytotoxicity and mutagenicity of coal oi
Cytotoxicity and mutagenicity of coal oils in the CHO/HGPRT assay
โœ Demarini, David M. ;Brimer, Patricia A. ;Hsie, Abraham W. ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Wiley (John Wiley & Sons) โš– 637 KB

We used the Chinese hamster ovary cell/hypoxanthine-guanine phosphoribosyl-transferase (CHO/HGPRT) assay to determine the cytotoxicity and mutagenicity of a crude coal oil, the neutral fraction of this crude, and the following three subfractions of the neutral fraction: aliphatic, neutral polar, and