Gene knockdown by RNAi in the pea aphidAcyrthosiphon pisum

✍ Scribed by Stéphanie Jaubert-Possamai; Gaël Le Trionnaire; Joël Bonhomme; Georges K Christophides; Claude Rispe; Denis Tagu

• Book ID: 104497623
• Publisher: BioMed Central
• Year: 2007
• Tongue: English
• Weight: 553 KB
• Volume: 7
• Category: Article
• ISSN: 1472-6750
• DOI: 10.1186/1472-6750-7-63

✦ Synopsis

Background

RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner.

Results

Here, we described the development of RNAi by micro-injection of double-stranded RNA (dsRNA) in the pea aphid Acyrthosiphon pisum. Injection of dsRNA into whole aphid body induced the silencing of two marker genes with different expression patterns: the ubiquitously expressed Ap-crt genes encoding a calreticulin and the gut specific Ap-cath-L gene encoding a cathepsin-L. Time-course analysis of the silencing showed similar temporal patterns for both genes: inhibition started at 1 day after injection, reached its maximum at 5 days and stopped at 7 days. A comparable 40% decrease of gene expression was observed for Ap-crt and Ap-cath-L.

Conclusion

The pea aphid is the first Hemipteran insect for which genome sequence will be available soon. The gene knockdown technique developed in this study will be an essential post-genomic tool for further investigations in aphidology.