Gene expression using xenopus oocytes
โ Scribed by Joel D. Richter
- Book ID
- 103978351
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 515 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0958-1669
No coin nor oath required. For personal study only.
โฆ Synopsis
Xenopus oocytes have been used as a surrogate genetic system for the study of many facets of gene expression. Some recent salient examples where injected oocytes proved to be indispensable for the analysis of transcription, translation, RNA/protein transport, and ion channel formation are described.
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We have explored the potential for cloning novel neu-INTRODUCTION rotrophic factor cDNAs via assay of neurotrophic activities following expression in Xenopus oocytes. In this report, we describe the successful application of the method to tract rat ciliary neurotrophic factor (CNTF) activity from mR