✦ LIBER ✦
Gene expression under the microscope recent advances in in situ hybridisation: 26th and 27th September, 1990, Royal Society of Medicine, London
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 45 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0009-3084
No coin nor oath required. For personal study only.
✦ Synopsis
This course of lectures and practical demonstrations has been designed to illustrate the principles, practical problems and applications of in situ hybridisation. Topics will include: riboprobes, oligonucleotide and DNA probes, probe construction; tissue collection and preparation; optimising prehybridisation and hybridisation conditions; choice of radiolabelled or biotiynlated probes; visualisation using autoradiography or chromogenic methods; combination with immunocytochemistry to provide a "functional morphology"; quantification of results using computer assisted image analysis; and applications to the study of neoplasia and endocrine pituitary manipulations.